Journal: International Journal of Molecular Sciences

Article Title: Structural Antitumoral Activity Relationships of Synthetic Chalcones

doi: 10.3390/ijms10010221

Figure Lengend Snippet: Effects of chalcones on the growth of HepG2 hepatome cells after 24 h Incubation. The results of studies are expressed as mean values ±SD from three separate experiments (-p<0.05).

Article Snippet: The HepG2 hepatocellular carcinoma cell line (HB 8065; American Type Culture Collection), derived from a human hepatoblastoma [ ], were maintained in Dulbeccós modified Eaglés (DMEM) with 10% heat-inactivated fetal bovine serum (FBS) at 37°C with 5% CO 2.

Techniques: Incubation

Journal: International Journal of Molecular Sciences

Article Title: Structural Antitumoral Activity Relationships of Synthetic Chalcones

doi: 10.3390/ijms10010221

Figure Lengend Snippet: Effects of chalcones on the DNA fragmentation pattern analysis by agarose gel electrophoresis of HepG2 cells after 24 h incubation.

Article Snippet: The HepG2 hepatocellular carcinoma cell line (HB 8065; American Type Culture Collection), derived from a human hepatoblastoma [ ], were maintained in Dulbeccós modified Eaglés (DMEM) with 10% heat-inactivated fetal bovine serum (FBS) at 37°C with 5% CO 2.

Techniques: Agarose Gel Electrophoresis, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Structural Antitumoral Activity Relationships of Synthetic Chalcones

doi: 10.3390/ijms10010221

Figure Lengend Snippet: Nuclear morphology of HepG2 cells stained with ethidium bromide. The cells were incubated in the absence (control) or presence of 100 μM chalcone 1 after 24 h incubation.

Article Snippet: The HepG2 hepatocellular carcinoma cell line (HB 8065; American Type Culture Collection), derived from a human hepatoblastoma [ ], were maintained in Dulbeccós modified Eaglés (DMEM) with 10% heat-inactivated fetal bovine serum (FBS) at 37°C with 5% CO 2.

Techniques: Staining, Incubation, Control

Journal: International Journal of Molecular Sciences

Article Title: Structural Antitumoral Activity Relationships of Synthetic Chalcones

doi: 10.3390/ijms10010221

Figure Lengend Snippet: Chalcones induces activation of caspase-9. HepG2 cells treated with chalcone (200 μM) were analyzed for caspase activity after 24 h incubation. The results of studies are expressed as mean values ±SD from four separate experiments (-P<0.05).

Article Snippet: The HepG2 hepatocellular carcinoma cell line (HB 8065; American Type Culture Collection), derived from a human hepatoblastoma [ ], were maintained in Dulbeccós modified Eaglés (DMEM) with 10% heat-inactivated fetal bovine serum (FBS) at 37°C with 5% CO 2.

Techniques: Activation Assay, Activity Assay, Incubation

Journal: International Journal of Molecular Sciences

Article Title: Structural Antitumoral Activity Relationships of Synthetic Chalcones

doi: 10.3390/ijms10010221

Figure Lengend Snippet: Chalcone induced apoptosis of HepG2 cells depends on the activation of caspases. Cells were treated with zVAD (50 μM) for 2 h and then incubated with chalcones 1 and 3 (200 μM) for 24 h. (cases marked by +). When the cells were incubated in absence of inhibitor (case -), typical laddering indicative of apoptosis was observed. Only representative compounds 1 and 3 are shown, but similar activity were found for all compounds tested.

Article Snippet: The HepG2 hepatocellular carcinoma cell line (HB 8065; American Type Culture Collection), derived from a human hepatoblastoma [ ], were maintained in Dulbeccós modified Eaglés (DMEM) with 10% heat-inactivated fetal bovine serum (FBS) at 37°C with 5% CO 2.

Techniques: Activation Assay, Incubation, Activity Assay

Journal:

Article Title: Reduction of NO Synthase Expression and Tumor Necrosis Factor Alpha Production in Macrophages by Amphotericin B Lipid Carriers

doi: 10.1128/AAC.45.2.553-562.2001

Figure Lengend Snippet: Immunostimulating effect of AMB on TNF-α production. Macrophages (105 cells per well) were cultured with various combinations of immunostimulants after an activation phase of 5 h. TNF-α production was determined as described in Materials and Methods. (A) TNF-α production as a function of IFN-γ concentration. □, IFN-γ alone; ○, IFN-γ with AMB (0.3 μg/ml); ●, IFN-γ with LPS (5 μg/ml); ■, IFN-γ with AMB (0.3 μg/ml) and LPS (5 μg/ml). (B) TNF-α production as a function of AMB concentration. ■, AMB alone; ●, AMB with LPS (5 μg/ml); ○, AMB with IFN-γ (5 IU/ml); □, AMB with IFN-γ (5 IU/ml) and LPS (5 μg/ml).

Article Snippet: Similar results concerning TNF-α production were obtained with AmBisome in vitro ( 31 ) and in vivo ( 3 ).

Techniques: Cell Culture, Activation Assay, Concentration Assay

Journal:

Article Title: Reduction of NO Synthase Expression and Tumor Necrosis Factor Alpha Production in Macrophages by Amphotericin B Lipid Carriers

doi: 10.1128/AAC.45.2.553-562.2001

Figure Lengend Snippet: Effects of lipid formulations of AMB on TNF-α production. Macrophages (105 per well) were cultured with IFN-γ (20 IU/ml) and various concentrations and formulations of AMB. After an activation phase of 5 h, TNF-α production was determined as described in Materials and Methods. (A) Liposomes. Filled bars, AMB; open bars, AmBisome, cross-hatched bars, ampholiposomes. (B) Complexes. Filled bars, AMB; open bars, Abelcet; dotted bars, LC-AMB; cross-hatched bars, Amphotec.

Article Snippet: Similar results concerning TNF-α production were obtained with AmBisome in vitro ( 31 ) and in vivo ( 3 ).

Techniques: Cell Culture, Activation Assay

Journal:

Article Title: Reduction of NO Synthase Expression and Tumor Necrosis Factor Alpha Production in Macrophages by Amphotericin B Lipid Carriers

doi: 10.1128/AAC.45.2.553-562.2001

Figure Lengend Snippet: Influence of the lipid on the activation of murine peritoneal macrophages a

Article Snippet: Similar results concerning TNF-α production were obtained with AmBisome in vitro ( 31 ) and in vivo ( 3 ).

Techniques: Activation Assay